I-1, ii-reagents zokuhlalutya kunye neendlela zokuphendula

I-arhente yokunciphisa kunye ne-ejenti yokufihla

IVithamin C idla ngokusetyenziswa njengesixhobo sokunciphisa esomeleleyo elabhoratri ukuthoba izinto ezixubene ne-oxidizing (ezifana ne-iodine esisiseko), kwaye ukuncitshiswa kwayo kunokuqinisekiswa ngokubonakalayo ngemifuniselo yokuphela kwe-iodine.

Kwi-complexometric titration, i-vitamin C inokusebenza njenge-mask agent ukuphelisa ukuphazamiseka kwee-ion zetsimbi ekubhaqweni.

Uphando kwiRedox Reaction

Ukumiselwa kobungakanani bomxholo wevithamin C ngokusabela kokunciphisa i-oxidation kunye ne-iodine esisiseko (efana nendlela ye-iodometric) isetyenziswa ngokubanzi kuhlalutyo lokutya kunye neesampulu zebhayoloji.

2, Itekhnoloji yokufumanisa kunye neemeko zesicelo

Ukuphuhliswa kweendlela zokufumanisa ubungakanani

Uvavanyo lweBiomedical: usebenzisa i-UV spectrophotometry, i-high-performance liquid chromatography (HPLC), njl., Ukuchonga ngokuchanekileyo umxholo we-vitamin C kwi-serum kunye nezicubu, ukuncedisa ukuxilongwa kwekliniki (njenge-scurvy).

Uhlalutyo lokutya kunye nezithambiso: Vavanya uzinzo kunye nexabiso lesondlo sevithamin C kwiziqhamo, imifuno, kunye nezithambiso usebenzisa icopper oxidation colorimetric okanye iindlela zolwelo lwechromatography.

Uphando lwePhysiology yezityalo

Khangela umxholo we-vitamin C kwiithishu zezityalo ukuvavanya impembelelo yoxinzelelo lwendalo (njengembalela, ukungcola kwesinyithi esinzima) kwiinkqubo ze-antioxidant zezityalo.

3. Inkqubo yokulungiselela kunye nophuhliso loqulunqo

Ukuphuculwa kwenkqubo yokulungiselela ilabhoratri

Ukwamkela itekhnoloji yokumisa umkhenkce ukuphucula uzinzo lwevithamin C reagents, kunye nokususa ukungcola ngokusebenzisa i-activated carbon pretreatment ukuqinisekisa ukuchaneka kokubhaqwa.

Phuhlisa iifom zedosi ezintsha ezinje nge-enteric coated formulations kunye neegranules ukomeleza ukufumaneka kwe-bioavailability yevithamin C.

Ukuveliswa kweemveliso eziqhelekileyo kunye neekiti ze-reagent

Iikiti ze-reagent ezisemgangathweni (ezifana nezixhobo zokufumanisa i-vitamin C) ezidityaniswe ne-algorithms yengqondo eyenziweyo yenza uhlalutyo olusebenzayo lweesampuli ezinkulu.

4. Ukuqinisekiswa kovavanyo kunye nokulawula umgangatho

Indlela yokuqinisekisa

Qinisekisa ukuchaneka kwendlela yokufumanisa ngokusebenzisa imifuniselo yokubuyisela i-spiked (izinga lokubuyisela le-95.6% ~ 101.0%).

Thelekisa iindlela ezahlukeneyo ezifana ne-2,4-dinitrophenylhydrazine colorimetric method kunye nendlela ye-iodometric ukuqinisekisa ukuthembeka kweziphumo.

Ukulawulwa kwezinto zokuphazamiseka

Lungiselela iimeko zokusabela (ezifana ne-pH kunye nobushushu) ukunciphisa ukuphazamiseka kwezinye izinto zokunciphisa (ezifana ne-glutathione).

Isishwankathelo: I-Vitamin C inomsebenzi we-reagent kunye neepropati zophando kwibhubhoratri. Itekhnoloji yokufumanisa itekhnoloji, inkqubo yokulungiselela, kunye nophando lwendlela yokusabela ibonelela ngenkxaso yobugcisa ephambili kwi-biomedical, isayensi yokutya, kunye neminye imimandla.

http://www.jvvw.cn/ascorbic-acid-is-also-known-as-vitamin/