1. Ama-reagents okuhlaziya kanye nezindlela zokusabela

I-ejenti yokunciphisa kanye ne-ejenti yokufihla

I-Vitamin C ivamise ukusetshenziswa njenge-ejenti yokunciphisa eqinile elabhorethri ukuze kuncishiswe izinto ezikhipha i-oxidizing (njenge-iodine eyisisekelo), futhi ukuncipha kwayo kungaqinisekiswa ngokubukeka ngokuhlolwa kokufiphala kwe-iodine.

Ku-complexometric titration, uvithamini C angasebenza njenge-ejenti yokufihla ukuze asuse ukuphazamiseka kwama-ion ensimbi ekubonweni.

Ucwaningo nge-Redox Reaction

Ukunqunywa kwenani lokuqukethwe kwevithamini C ngokusabela kokunciphisa i-oxidation nge-iodine eyisisekelo (njengendlela ye-iodometric) kusetshenziswa kakhulu ekuhlaziyeni ukudla namasampula ebhayoloji.

2, Ubuchwepheshe bokubona kanye nezimo zohlelo lokusebenza

Ukuthuthukiswa kwezindlela zokuthola inani

Ukuhlolwa kwe-Biomedical: kusetshenziswa i-UV spectrophotometry, i-high-performance liquid chromatography (HPLC), njll., ukuze kutholwe ngokunembile okuqukethwe kwevithamini C ku-serum nezicubu, okusiza ekuxilongweni komtholampilo (okufana ne-scurvy).

Ukuhlaziywa kokudla nezimonyo: Hlola ukuzinza kanye nevelu yomsoco kavithamini C ezithelweni, imifino, nezimonyo usebenzisa izindlela ze-copper oxidation colorimetric noma ze-chromatography ewuketshezi.

Ucwaningo lwePhysiology yezitshalo

Thola okuqukethwe kukavithamini C ezicutshini zezitshalo ukuze uhlole umthelela wokucindezeleka kwendawo (njengesomiso, ukungcoliswa kwensimbi enzima) ezinhlelweni zokulwa nezitshalo.

3, Inqubo yokulungiselela kanye nokuthuthukiswa kokwakhiwa

Ukuthuthukisa inqubo yokulungiselela ilabhorethri

Ukwamukela ubuchwepheshe bokumisa iqhwa ukuze kuthuthukiswe ukuzinza kwama-reagents kavithamini C, kanye nokukhipha ukungcola ngokusebenzisa i-activated carbon pretreatment ukuqinisekisa ukunemba kokutholakala.

Dala amafomu emithamo amasha anjengama-enteric coated compounds nama-granules ukuze kuthuthukiswe ukutholakala kwe-bioavailability kavithamini C.

Ukukhiqizwa kwemikhiqizo ejwayelekile kanye nama-reagent kits

Amakhithi ama-reagent ajwayelekile (njengamakhithi okuthola uvithamini C) ahlanganiswe nama-algorithms obuhlakani bokwenziwa anika amandla ukuhlaziya okusebenzayo kwamasampuli amakhulu.

4. Ukuqinisekisa kokuhlola nokulawula ikhwalithi

Ukuqinisekiswa kwendlela

Qinisekisa ukunemba kwendlela yokuthola ngokusebenzisa izivivinyo zokuphinda uthole kabusha (izinga lokutakula elingu-95.6%~101.0%).

Qhathanisa izindlela ezihlukahlukene ezifana ne-2,4-dinitrophenylhydrazine colorimetric indlela kanye nendlela ye-iodometric ukuqinisekisa ukwethembeka kwemiphumela.

Ukulawulwa kwezinto eziphazamisayo

Lungiselela izimo zokusabela (njenge-pH nezinga lokushisa) ukuze unciphise ukuphazamiseka kwezinye izinto ezinciphisayo (njenge-glutathione).

Isifinyezo: I-Vitamin C inokubili ukusebenza kwe-reagent kanye nezakhiwo zento yocwaningo elabhorethri. Ubuchwepheshe bayo bokutholwa, inqubo yokulungiselela, kanye nocwaningo lwendlela yokusabela kunikeza ukusekelwa okubalulekile kwezobuchwepheshe kwe-biomedical, isayensi yokudla, neminye imikhakha.

http://www.jvvw.cn/ascorbic-acid-is-also-known-as-vitamin/